In Vitro Antiprotozoal Activities and Cytotoxicity of Selected Sudanese Medicinal Plants
Ahmed S. Kabbashi,
El-badri E. Osman,
Mohamed I. Garbi,
Ibrahim F. Ahmed,
Mahmmoud S. Saleh,
Ali M. Badri,
Ahmed A. Elshikh,
Nadir Abuzeid,
Waleed S. Koko,
Mahmoud M. Dahab
Issue:
Volume 3, Issue 2, March 2017
Pages:
6-13
Received:
12 May 2017
Accepted:
24 May 2017
Published:
23 August 2017
Abstract: Entamoeba histolytica is ranked third on the list of parasitic protozoan infections leading to death behind malaria and schistosomiasis. It is estimated also up to 200 millions of people are chronically infected with Giardia lamblia globally. Metronidazole is the drug currently widely used and recommended in the treatment of amoebiasis and giardiasis. However; sometimes it causes adverse effects such as myoplasia, neuralgia, allergic dermatitis, and others. The in vitro antiprotozoal activities of some selected Sudanese medicinal plants (Acacia nilotica subsp. nilotica, Adansonia digitata, Cyperus rotundus and Nigella sativa) were determined against Entamoeba histolytica and Giardia lamblia by employing the sub-culture method. The mammalian cytotoxicity of the investigated plants against normal Vero cell line was determined by applying MTT [(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] method. All plants examined 100% inhibition at a concentration 500 μg/ml after 96 h; this was compared with Metronidazole which gave 95% inhibition at concentration 312.5 μg/ml at the same time against Entamoeba histolytica and Giardia lamblia. In addition, cytotoxicity (MTT-assay) of these plants against normal Vero cell line which verified the safety of the examined extracts with an IC50 less 100 μg/ml. These studies prove the potent activity of extracts against E. histolyica and G. lamblia trophozoites in vitro with verified safety evidence for use.
Abstract: Entamoeba histolytica is ranked third on the list of parasitic protozoan infections leading to death behind malaria and schistosomiasis. It is estimated also up to 200 millions of people are chronically infected with Giardia lamblia globally. Metronidazole is the drug currently widely used and recommended in the treatment of amoebiasis and giardias...
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Asymptomatic Hepatitis C in Patient with Oral Lichen Planus: A Case Report
Suresh Kumar Sachdeva,
Purnendu Rout,
Debahuti Patnaik,
Ashok Galav
Issue:
Volume 3, Issue 2, March 2017
Pages:
14-17
Received:
3 June 2017
Accepted:
22 June 2017
Published:
18 October 2017
Abstract: Lichen planus (LP) is a chronic inflammatory condition that affects the oral mucous membrane/skin with a variety of clinical presentations. Oral Lichen Planus (OLP) is considered to be associated with numerous systemic conditions one of which includes hepatitis C virus (HCV) infection. The geographic variation of the hepatitis C prevalence proved to be an important factor influencing the statistical results of the studies analyzing the association of the oral plan lichen with the hepatitis C virus. LP patients have about a fivefold higher risk than the controls of being HCV seropositive, with geographic variability, being most prevalent in the region of Japan, Mediterranean countries and the USA. Treatment outcomes in patients with oral lichen planus associated with chronic hepatitis C virus are often unsatisfactory compared to patients suffering from idiopathic oral lichen planus. Also, the evolution of oral lesions is often fluctuating, with repeated periods of relapse according to the degree of liver function decompensation. Herewith, a case of oral lichen planus in a female patient is reported, which on investigation revealed hepatitis C antibodies.
Abstract: Lichen planus (LP) is a chronic inflammatory condition that affects the oral mucous membrane/skin with a variety of clinical presentations. Oral Lichen Planus (OLP) is considered to be associated with numerous systemic conditions one of which includes hepatitis C virus (HCV) infection. The geographic variation of the hepatitis C prevalence proved t...
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