Volume 6, Issue 3, September 2020, Page: 60-67
Antiproliferative Effects of Ferula assa-foetida’s Extract on PC12 and MCF7 Cancer Cells
Mina Abroudi, Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
Amir Ganji Fard, Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
Ghazaleh Dadashizadeh, Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
Omid Gholami, Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran; Department of Pharmacology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
Davood Mahdian, Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran; Department of Pharmacology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
Received: Aug. 3, 2020;       Accepted: Aug. 17, 2020;       Published: Oct. 30, 2020
DOI: 10.11648/j.ijbecs.20200603.12      View  54      Downloads  39
Abstract
Background: Ferula assa-foetida is a herbaceous plant of the Umbelliferae family having a broad spectrum of biological activities such as antiseptic, antibacterial, anti-inflammation, and, anti-tumor activity against a wide range of cancers. Among these features, antitumor activity has become more important in recent years and it still demands more investigations to address the underlying mechanisms. Purpose: This current study was conducted to evaluate the anti-proliferative effect of Ferula assa-foetida on PC12 and MCF7 cells as well as examining its mechanisms. Materials and Methods: Cells were cultured in DMEM medium with 10% fetal bovine serum, 100 units/ml penicillin, and 100 µg/ml streptomycin. Cells incubated with different concentrations of the ethanolic extract of Ferula assa-foetida. Notably, cytotoxicity and apoptosis assay were measured by MTT and PI staining, respectively. Results: The MTT results showed that the ethanolic extract of Ferula assa-foetida in concentrations of 10, 7, 5, and 2.5 μM on both PC12 and MCF7 cells had a significant effect in cell viability and apoptosis induction in comparison to control group. Conclusion: In this study, it was determined that Ferula assa-fetida through the induction of apoptosis prevented the growth of PC12 and MCF7 cells and made a reduction in cell viability with different concentrations in a time- and dose-dependent manner. However, more studies are needed to reveal the mechanisms of Ferula assa-foetida’s extract in apoptosis induction.
Keywords
Ferula assa-foetida, Apoptosis, Cytotoxicity, Cancer, MCF7, PC12
To cite this article
Mina Abroudi, Amir Ganji Fard, Ghazaleh Dadashizadeh, Omid Gholami, Davood Mahdian, Antiproliferative Effects of Ferula assa-foetida’s Extract on PC12 and MCF7 Cancer Cells, International Journal of Biomedical Engineering and Clinical Science. Vol. 6, No. 3, 2020, pp. 60-67. doi: 10.11648/j.ijbecs.20200603.12
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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