Volume 5, Issue 3, September 2019, Page: 40-44
Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics
Abdoulaye Diop, Bacteriology and Virology Laboratory, Le Dantec Teaching Hospital, Dakar, Senegal
Assane Dieng, Bacteriology and Virology Laboratory, Le Dantec Teaching Hospital, Dakar, Senegal
Abdoulaye Seck, Medical Virology Unit, Institute Pasteur, Dakar, Senegal
Amary Fall, Medical Virology Unit, Institute Pasteur, Dakar, Senegal
Amadou Diop, Bacteriology and Virology Laboratory, Le Dantec Teaching Hospital, Dakar, Senegal
Djibril Boiro, Paediatric Unit, Abass NDAO Teaching Hospital, Dakar, Senegal
Jean Baptisse Niokhor Diouf, Paediatric Unit, Roi Baudouin Hospital, Dakar, Senegal
Modou Gueye, Paediatric Unit, Abass NDAO Teaching Hospital, Dakar, Senegal
Mbayame Niang, Medical Virology Unit, Institute Pasteur, Dakar, Senegal
Makhtar Camara, Bacteriology and Virology Laboratory, Le Dantec Teaching Hospital, Dakar, Senegal
Cheikh Saad Bouh Boye, Bacteriology and Virology Laboratory, Le Dantec Teaching Hospital, Dakar, Senegal
Received: Feb. 20, 2019;       Accepted: Apr. 10, 2019;       Published: Oct. 16, 2019
DOI: 10.11648/j.ijbecs.20190503.12      View  39      Downloads  10
Abstract
Haemophilus influenzae is a bacterium that can cause severe infections, occurring mostly in infants and children younger than five years of age. Antibiotic treatment may cause the emergence of resistant H. influenzae strains, particularly ampicillin-resistant strains. Antimicrobial resistance is a public health threat worldwide, particularly in the developing world. H. influenzae strains have been isolated from broncho-alveolar lavages (BALs), nasopharyngeal swabs, and otitis media from children in two paediatric centers at Dakar, Senegal. Antibiotic susceptibility testing was carried out using strips E Test ®t method that provides the ability to precisely determine the minimum inhibitory concentration (MIC). A total of 16 H. influenzae strains have been isolated and identified, including 16.7% of ampicillin-resistant patterns (all β-lactamase-negative), 9.4% of the isolates were resistant to cefaclor (MIC90 = 16 µg/ml) while 100% were susceptible to cefixime to (MIC90 = 0.38 µg/ml). Interestingly, fluoroquinolones were fully active with very low MIC90. Macrolide were still active against H. influenzae isoles although with higher MIC azitrhomycin MIC90= 3µg/ml, clarithromycin MIC90= 12µg/ml. Ampicillin-resistance has become increasingly reported in H. influenzae, suggesting a continuous laboratory based surveillance for antimicrobial resistance pattern for a better management of acute respiratory infections, particularly in low incomes settings.
Keywords
Respiratory Tract Infections, Haemophilus influenzae, Ampicillin Resistance
To cite this article
Abdoulaye Diop, Assane Dieng, Abdoulaye Seck, Amary Fall, Amadou Diop, Djibril Boiro, Jean Baptisse Niokhor Diouf, Modou Gueye, Mbayame Niang, Makhtar Camara, Cheikh Saad Bouh Boye, Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics, International Journal of Biomedical Engineering and Clinical Science. Vol. 5, No. 3, 2019, pp. 40-44. doi: 10.11648/j.ijbecs.20190503.12
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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