Background: Carbapenemase-producing Klebsiella pneumoniae (CPKP) has emerged as a major global concern due to extensive antimicrobial resistance, limited therapeutic options, and significant mortality rates. This study aimed to characterize the molecular mechanisms of carbapenem resistance and assess the antibiotic susceptibility patterns of CPKP isolates collected from Tay Nguyen Regional General Hospital, Vietnam. Methods: Seventy-nine non-duplicate K. pneumoniae isolates resistant to at least one carbapenem were analyzed using the modified carbapenem inactivation method (mCIM) and real-time PCR to detect blaKPC, blaNDM, blaVIM and blaOXA-48-like genes. Antimicrobial susceptibility was tested using the disk diffusion method following CLSI 2023 guidelines. Results: Among the 79 isolates, 62 (78.5%) were carbapenemase producers. The most prevalent carbapenemase gene was blaNDM (65.6%), followed by blaOXA-48-like (48.4%), blaKPC (35.9%) and blaVIM (32.8%). Co-harboring of multiple genes was common blaNDM+OXA-48-like (29.7%), blaKPC+NDM (21.9%). Resistance rates exceeded 90% for β-lactams, cephalosporins and quinolones, while all isolates remained susceptible to colistin. Conclusions: The predominance of blaNDM and blaOXA-48-like genes highlights the evolving resistance landscape in Vietnam. Strengthening molecular surveillance, infection control and rational antibiotic use is essential to prevent the spread of multidrug-resistant pathogens.
| Published in | International Journal of Biomedical Engineering and Clinical Science (Volume 11, Issue 4) |
| DOI | 10.11648/j.ijbecs.20251104.13 |
| Page(s) | 73-79 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2025. Published by Science Publishing Group |
Carbapenemase, Antibiotic Resistance, Klebsiella pneumoniae, Resistance Genes
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APA Style
Nie, H. N., Thuy, L. K., Loan, N. T. K., Tuoi, N. H. (2025). Detection of Carbapenemase Genes in Carbapenemase-producing Klebsiella pneumoniae Isolated from Tay Nguyen Regional General Hospital, Vietnam. International Journal of Biomedical Engineering and Clinical Science, 11(4), 73-79. https://doi.org/10.11648/j.ijbecs.20251104.13
ACS Style
Nie, H. N.; Thuy, L. K.; Loan, N. T. K.; Tuoi, N. H. Detection of Carbapenemase Genes in Carbapenemase-producing Klebsiella pneumoniae Isolated from Tay Nguyen Regional General Hospital, Vietnam. Int. J. Biomed. Eng. Clin. Sci. 2025, 11(4), 73-79. doi: 10.11648/j.ijbecs.20251104.13
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Download@article{10.11648/j.ijbecs.20251104.13,
author = {H Nuong Nie and Le Ka Thuy and Nguyen Thi Kim Loan and Nguyen Hong Tuoi},
title = {Detection of Carbapenemase Genes in Carbapenemase-producing Klebsiella pneumoniae Isolated from Tay Nguyen Regional General Hospital, Vietnam},
journal = {International Journal of Biomedical Engineering and Clinical Science},
volume = {11},
number = {4},
pages = {73-79},
doi = {10.11648/j.ijbecs.20251104.13},
url = {https://doi.org/10.11648/j.ijbecs.20251104.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbecs.20251104.13},
abstract = {Background: Carbapenemase-producing Klebsiella pneumoniae (CPKP) has emerged as a major global concern due to extensive antimicrobial resistance, limited therapeutic options, and significant mortality rates. This study aimed to characterize the molecular mechanisms of carbapenem resistance and assess the antibiotic susceptibility patterns of CPKP isolates collected from Tay Nguyen Regional General Hospital, Vietnam. Methods: Seventy-nine non-duplicate K. pneumoniae isolates resistant to at least one carbapenem were analyzed using the modified carbapenem inactivation method (mCIM) and real-time PCR to detect blaKPC, blaNDM, blaVIM and blaOXA-48-like genes. Antimicrobial susceptibility was tested using the disk diffusion method following CLSI 2023 guidelines. Results: Among the 79 isolates, 62 (78.5%) were carbapenemase producers. The most prevalent carbapenemase gene was blaNDM (65.6%), followed by blaOXA-48-like (48.4%), blaKPC (35.9%) and blaVIM (32.8%). Co-harboring of multiple genes was common blaNDM+OXA-48-like (29.7%), blaKPC+NDM (21.9%). Resistance rates exceeded 90% for β-lactams, cephalosporins and quinolones, while all isolates remained susceptible to colistin. Conclusions: The predominance of blaNDM and blaOXA-48-like genes highlights the evolving resistance landscape in Vietnam. Strengthening molecular surveillance, infection control and rational antibiotic use is essential to prevent the spread of multidrug-resistant pathogens.},
year = {2025}
}
TY - JOUR T1 - Detection of Carbapenemase Genes in Carbapenemase-producing Klebsiella pneumoniae Isolated from Tay Nguyen Regional General Hospital, Vietnam AU - H Nuong Nie AU - Le Ka Thuy AU - Nguyen Thi Kim Loan AU - Nguyen Hong Tuoi Y1 - 2025/12/29 PY - 2025 N1 - https://doi.org/10.11648/j.ijbecs.20251104.13 DO - 10.11648/j.ijbecs.20251104.13 T2 - International Journal of Biomedical Engineering and Clinical Science JF - International Journal of Biomedical Engineering and Clinical Science JO - International Journal of Biomedical Engineering and Clinical Science SP - 73 EP - 79 PB - Science Publishing Group SN - 2472-1301 UR - https://doi.org/10.11648/j.ijbecs.20251104.13 AB - Background: Carbapenemase-producing Klebsiella pneumoniae (CPKP) has emerged as a major global concern due to extensive antimicrobial resistance, limited therapeutic options, and significant mortality rates. This study aimed to characterize the molecular mechanisms of carbapenem resistance and assess the antibiotic susceptibility patterns of CPKP isolates collected from Tay Nguyen Regional General Hospital, Vietnam. Methods: Seventy-nine non-duplicate K. pneumoniae isolates resistant to at least one carbapenem were analyzed using the modified carbapenem inactivation method (mCIM) and real-time PCR to detect blaKPC, blaNDM, blaVIM and blaOXA-48-like genes. Antimicrobial susceptibility was tested using the disk diffusion method following CLSI 2023 guidelines. Results: Among the 79 isolates, 62 (78.5%) were carbapenemase producers. The most prevalent carbapenemase gene was blaNDM (65.6%), followed by blaOXA-48-like (48.4%), blaKPC (35.9%) and blaVIM (32.8%). Co-harboring of multiple genes was common blaNDM+OXA-48-like (29.7%), blaKPC+NDM (21.9%). Resistance rates exceeded 90% for β-lactams, cephalosporins and quinolones, while all isolates remained susceptible to colistin. Conclusions: The predominance of blaNDM and blaOXA-48-like genes highlights the evolving resistance landscape in Vietnam. Strengthening molecular surveillance, infection control and rational antibiotic use is essential to prevent the spread of multidrug-resistant pathogens. VL - 11 IS - 4 ER -
Tay Nguyen Regional General Hospital, Dak Lak, Vietnam
Department of Anesthesia and Critical Care, Hanoi Medical University, Hanoi, Vietnam
